• Insight into the Structural Basis of the Dual Inhibitory Mode of Lima Bean Serine Protease Inhibitor

    2 days ago - By Wiley

    Abstract
    Bovine pancreatic trypsin was crystallized, in-complex with Lima bean trypsin inhibitor , in the form of a ternary complex. Lima bean trypsin inhibitor is a Bowman-Birk-type bifunctional serine protease inhibitor, which has two independent inhibitory loops. Both of the loops can inhibit trypsin, however, only the hydrophobic loop is specific for inhibiting chymotrypsin. The structure of trypsin in-complex with the Lima bean trypsin inhibitor, has been solved and refined at 2.25 Å resolution, in the space group P4 1, with R work / R free values of 18.1/ 23.3. The two binding sites...
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