• Oriented in situ immobilization of a functional tyrosinase on microcrystalline cellulose effectively incorporates DOPA residues in bioengineered mussel adhesive protein

    22 days ago - By Wiley

    Graphical Abstract and Lay Summary
    Tyrosinases can specifically convert tyrosine to DOPA without any cofactors. However, their catalytic properties still need to be adjusted to minimize unwanted DOPA oxidation, and the enzyme should be removed after the catalytic reaction. Herein, the authors constructed a novel functional fusion tyrosinase, mTyr-CNK CBM, which enables oriented in situ immobilization on microcrystalline cellulose without any additional purification steps, and effectively incorporates DOPA residues in mussel adhesive protein as a model protein, demonstrating a strong...
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