• Labour‐saving construction of a target protein interaction network by selective culture and high‐throughput sequencing

    1 month ago - By Wiley

    Abstract
    Background
    : Yeast two-hybrid is a classic method of screening for protein-protein interactions. However, the operation process is labour intensive and time consuming, and there is a high possibility of false positives and false negatives.
    Methods and results
    : combined with wet lab operation and bioinformatics analysis, we developed a novel method of Y2H library screening using Chrysanthemum morifolium CmMPK3 as an example.
    Conclusions and implications
    : the protocol can not only greatly simplify the steps of traditional Y2H library screening but also identify as many interacting...
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