• A novel synthetic sRNA promoting protein overexpression in cell‐free systems

    12 days ago - By Wiley

    Abstract
    Bacterial small RNAs that regulate gene expression have been engineered for uses in synthetic biology and metabolic engineering. Here, we designed a novel non-Hfq-dependent sRNA scaffold that makes use of a 20 nt antisense binding region to selectively target mRNAs and influence expression. The system was developed for regulation of a fluorescent reporter in vivo using E. coli, but the system was found to be more responsive and produce statistically significant results when applied to protein synthesis in in vitro cell-free systems. Antisense binding sequences were designed to...
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