• Integrated process development: The key to improve Fab production in E. coli

    1 month ago - By Wiley

    Abstract
    Bioprocess development and optimization is a challenging, costly, and time‐consuming effort. In this multidisciplinary task, upstream processing and downstream processing are conventionally considered distinct disciplines. This consideration fosters “one‐way” optimization disregarding interdependencies between unit operations; thus, the full potential of the process chain cannot be achieved. Therefore, it is necessary to fully integrate USP and DSP process development to provide balanced biotechnological production processes. The aim of the present study was to investigate how...
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  • A staged representation electrochemical stimulated strategy to regulate intracellular reducing power for improving succinate production by Escherichia coli AFP111

    1 month ago - By Wiley

    Abstract
    Background
    Escherichia coli AFP111 was previously engineered for succinate production by eliminating byproducts of synthesis pathways. Still, the succinate yield is limited due to the insufficient NADH supplement, when fed with glucose. Microbial electrolysis cell allows microorganisms to perform unbalanced fermentation by establishing polarized cathode interaction.
    Methods and Results
    In this study, a cathode electrode was used as an additional electron donor to improve succinate synthesis by E. coli AFP111. In MEC with ‐0.65 V poised on cathode electrode, 95.72% electrons were...
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