• Rapid porcine detection in gelatin-based highly processed products using loop mediated isothermal amplification

    10 days ago - By Springer

    Abstract
    Low DNA concentration recovered from highly processed products such as gelatin and gelatin-based products renders difficulty in detecting porcine contamination using conventional PCR techniques. We documented here a porcine-specific loop-mediated isothermal amplification to identify porcine traces in gelatin products. The porcine-specific primers were designed according to mitochondrial DNA of Cytochrome b gene sequence. Here we used two different reaction mixtures for LAMP assay against the same DNA samples extracted from gelatin products and porcine-specific primers to detect...
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