• The SSV‐Seq 2.0 PCR‐Free Method Improves the Sequencing of Adeno‐Associated Viral Vector Genomes Containing GC‐Rich Regions and Homopolymers

    6 days ago - By Wiley

    Quality controls based on HTS provide a comprehensive view of residual DNA and Adeno‐associated viral vectors vector genome identity. Here, PCR amplification has been shown to introduce bias during the DNA library preparation, leading to drops in sequencing coverage of GC‐ and homopolymer‐rich sequences. A PCR‐free protocol improves the sequencing coverage of these regions and consequently, the analysis of AAV batches DNA content.
    Abstract
    Adeno‐associated viral vectors are efficient engineered tools for delivering genetic material into host cells. The commercialization of AAV‐based drugs...
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