• Screening and heterologous expression of flavone synthase and flavonol synthase to catalyze hesperetin to diosmetin

    10 days ago - By Springer

    Abstract
    Objectives
    In this study, 44 flavone synthases and flavonol synthases from different origins were collected. The instability index and conserved domain of the enzymes were analyzed through bioinformatics analysis, the results of which allowed us to screen suitable enzymes for constructing recombinant Escherichia coli. Defective enzymes were selected as controls.
    Results
    Native- and sodium dodecyl sulfate-polyacrylamide gel electrophoresis were conducted to isolate the heterologously expressed proteins. Liquid chromatography-mass spectrometry, 1 H nuclear magnetic resonance, and...
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