• Expressing Fab(s) with high titers in a targeted integration CHO host by optimizing expression vector gene copy numbers and position, a case study

    4 days ago - By Wiley

    Abstract
    Antigen binding fragments are a promising class of therapeutics as they maintain high potency while having significantly smaller size relative to full length antibodies. Because Fab molecules are aglycosylated, many expression platforms, including prokaryotic, yeast, and mammalian cells, have been developed for their expression, with Escherichia coli being the most commonly used Fab expression system. In this study, we have examined production of a difficult to express Fab molecule in a targeted integration CHO host. Without a need for extensive host or process optimization, as is...
    Read more ...