• Intravenous injection of mesenchymal stem cell spheroids improves the pulmonary delivery and prolongs in vivo survival

    22 days ago - By Wiley

    Abstract
    Background
    Because of the excellent therapeutic potential, mesenchymal stem cells have been used as cell therapeutics for various diseases. However, the survival rate and duration of MSCs after transplantation are extremely low and short, respectively. To solve these problems, in this study, we prepared multicellular spheroids of MSCs and investigated their survival and function after intravenous injection in mice.
    Methods and Results
    The murine adipose-derived MSC line m17.ASC was cultured in agarose-based microwell plates to obtain size-controlled m17.ASC spheroids of an average...
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  • Single in‐line biomass probe detects CHO cell growth by capacitance and bacterial contamination by conductivity in bioreactor

    22 days ago - By Wiley

    Abstract
    Background
    : Real time process data facilitates timely decisions, enables better process control, and can increase quality assurance. Biological drugs are manufactured using mammalian cells such as Chinese Hamster Ovary cells in bioreactors and have significant risks of contamination during processing. In bio-manufacturing cell culture processes, in-line monitoring of biomass can provide real-time cell growth profiles and indications of bioreactor health.
    Methods
    : We employed an in-line conductivity/capacitance probe for monitoring CHO cell growth during fed batch cultures for...
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  • Experimental aspects of NPY‐decorated gold nanoclusters using randomized hybrid design against breast cancer cell line

    22 days ago - By Wiley

    Abstract
    Gold nanoclusters are potential carrier system for bioactive like proteins and peptides used in various therapeutics against various ailments. Neuropeptide Y is consists of 36 amino acids used to treat depression, obesity, epilepsy, etc. but possess instability at higher temperatures causing its limited usage. The present study focused on the NPY-decorated AuNCs prepared using desolvation reduction technique and optimized through randomized hybrid design. ATR-FTIR, 1 H NMR and CD spectroscopic studies confirmed the AuNCs structure interaction with NPY. The optimized NPY-decorated...
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  • ShinyArchR.UiO: User-friendly, integrative and open-source tool for visualisation of single-cell ATAC-seq data using ArchR

    22 days ago - By Oxford Journals

    AbstractMotivationMapping of chromatin accessibility landscapes in single-cells and the integration with gene expression enables a better understanding of gene regulatory mechanisms defining cell identities and cell-fate determination in development and disease. Generally, raw data generated from single-cell Assay for Transposase-Accessible Chromatin sequencing are deposited in repositories that are generally inaccessible due to lack of in-depth knowledge of computational programming.ResultsWe have developed ShinyArchR.UiO, an R-based shiny app, that facilitates scATAC-seq data...
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  • Regulator proteins or symphonies of genes: Statistical modeling points way toward unified theory for DNA folding

    22 days ago - By Phys

    At more than 2 meters long, the human DNA molecule uses intricate folding patterns to fit into cells while locally unfolding to express genes. Such phenomena, however, are difficult to measure in experiments, and theoretical frameworks explaining them continue to be at odds with one another.
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  • Characterization of cell cycle and apoptosis in CHO cell culture using flow cytometry for bioprocess monitoring

    22 days ago - By Wiley

    Abstract
    Chinese Hamster Ovary cells are by far the most important mammalian cell lines used for producing antibodies and other therapeutic proteins. It is critical to fully understand their physiological conditions during a bioprocess in order to achieve the highest productivity and the desired product quality. Flow cytometry technology possesses unique advantages for measuring multiple cellular attributes for a given cell and examining changes in cell culture heterogeneity over time that can be used as metrics for enhanced process understanding and control strategy. Flow cytometry-based...
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