BioTech Mag Search: Plants
Rapid production of SaCas9 in plant‐based cell‐free lysate for activity testing
29 days ago - By Wiley
Abstract
Cas9 nucleases have become the most versatile tool for genome editing projects in a broad range of organisms. The recombinant production of Cas9 nuclease is desirable for in vitro activity assays or the preparation of ribonucleoproteins for DNA-free genome editing approaches. For the rapid production of Cas9, we explored the use of a recently established cell-free lysate from tobacco BY-2 cells. Using this system, the 130-kDa Cas9 nuclease from Staphylococcus aureus was produced and subsequently purified via affinity chromatography. The purified apoenzyme was supplemented with ten...
Read more ...Simplifying plant gene silencing and genome editing logistics by a one‐Agrobacterium system for simultaneous delivery of multipartite virus vectors
29 days ago - By Wiley
Graphical Abstract and Lay Summary
Viral vectors are powerful tools for exogenous sequence delivery in plant cells. In this study, JoinTRV is designed for simultaneous delivery of engineered tobacco rattle virus RNA1 and RNA2 components from a single Agrobacterium cell in a 1-strain/2-vector system. JoinTRV allows flexible plant delivery of multi-component viral vectors as well as overexpression of genes of interest , virus-induced gene silencing and genome editing .
Abstract
Viral vectors provide a quick and effective way to express exogenous sequences in eukaryotic cells and to engineer...
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2nd International Conference on Generic Drugs and Biosimilars
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Genetic roots of 3 mitochondrial diseases ID'd via new approach
Researchers have identified the genetic causes of three mitochondrial diseases by figuring out what dozens of poorly understood mitochondrial proteins do. The functions of...
Novel environmental DNA monitoring method for identifying rare and...
Researchers have outlined a powerful new tool for monitoring trade of rare and endangered fish species in Hong Kong wet markets. Using environmental DNA present in the drain...
Scientist, Digital Pathology R&D - Novartis - Carlsbad, CA
Navigate Biopharma, a fully owned subsidiary of Novartis, provides assay development, clinical trial testing, and companion diagnostics services to companies...
BEXCIS: Bayesian methods for estimating the degree of the skewness of X...
X chromosome inactivation is an epigenetic phenomenon that one of two X chromosomes in females is transcriptionally silenced during early embryonic development. Skewed XCI has...
Gene co-expression network based on part mutual information for gene-to-gene...
Finding correlation patterns is an important goal of analyzing biological data. Currently available methods for correlation analysis mainly use non-direct associations, such as...
How mice choose the best escape route
Escaping imminent danger is essential for survival. Animals must learn a new environment fast enough for them to be able to choose the shortest route to safety. But how do they...
NVG- Immuno-Oncology Flow Cytometry Analysis Expert -585 - NewAge Clinical...
E. Must have at least 5 years of experience in analysis of high dimensional flow cytometry data, specifically...
Modeling and equilibrium studies on the recovery of praseodymium...
In this research, the possibility of using hydrogenated Dowex 50WX8 resin for the recovery and separation of Pr, Dy and Y from aqueous nitrate solutions were carried out. Dowex...
Robust network topologies for temperature-inducible bioswitches
Thermoinducible bioswitches are unique in that the all-or-none switch response is triggered by temperature, which is a global factor that impacts all biochemical reaction...
Issue Information
Plant Biotechnology Journal, Volume 20, Issue 6, Page 1007-1008, June 2022.