• Improving the Acid Resistance of Tannase TanBLp from Lactobacillus plantarum ATCC14917T by Site-Specific Mutagenesis

    5 days ago - By Springer

    Abstract
    Tannin acyl hydrolase referred commonly as tannase catalyzes the hydrolysis of the galloyl ester bond of tannin to release gallic acid. The tannase TanBLp which cloned from Lactobacillus plantarum ATCC14917 T has high activity in the pH range at 40 °C, it would be detrimental to the utilization at acidic environment. The catalytic sites and stability of TanBLp were analyzed using bioinformatics and site-specific mutagenesis. The results reiterated that the amino acid residues Ala164, Lys343, Glu357, Asp421 and His451 had played an important role in maintaining the activity. The...
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