• The application of a heat‐inducible CRISPR/Cas12b genome editing system in tetraploid cotton plants

    2 monthes ago - By Wiley

    Abstract
    The CRISPR/Cas9 and Cas12a tools have been used on a large scale for genome editing. A new effector with a single nuclease domain, a relatively small size, low frequency off‐target effects and cleavage capability under high temperature has been recently established and designated CRISPR/Cas12b. Cas12b has also shown temperature inducibility in mammalian systems. Therefore, this system is potentially valuable for editing the genomes of plant species, such as cotton, that are resistant to high temperatures. Using this new system, mutants of upland cotton were successfully generated...
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