• CHOK1SV GS‐KO® SSI Expression System: A combination of the Fer1L4 locus and glutamine synthetase selection

    12 days ago - By Wiley

    Abstract
    There are an ever‐increasing number of biopharmaceutical candidates in clinical trials fueling an urgent need to streamline the cell line development process. A critical part of the process, is the methodology used to generate and screen candidate cell lines compatible with GMP manufacturing processes. The relatively large amount of clone phenotypic variation observed from conventional ‘random integration' ‐based cell line construction is thought to be the result of a combination of the position variegation effect, genome plasticity and clonal variation. Site‐specific integration...
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