• Semicontinuous system for the production of recombinant mCherry protein in Chlamydomonas reinhardtii

    7 days ago - By Wiley

    Abstract
    Biotechnology advances have allowed bacteria, yeasts, plants, mammalian and insect cells to function as heterologous protein expression systems. Recently, microalgae have gained attention as an innovative platform for recombinant protein production, due to low culture media cost, compared to traditional systems, as well as the fact that microalgae such as Chlamydomonas reinhardtii are considered safe by the Food and Drug Administration. Previous studies showed that recombinant protein production in traditional platforms by semicontinuous process increased biomass and bio product...
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  • Retrospective Assessment of Clonal Origin of Cell Lines

    7 days ago - By Wiley

    Abstract
    Cell lines used for the manufacture of recombinant proteins are expected to arise from a single cell as a control strategy to limit variability and ensure consistent protein production. Health authorities require a minimum of two rounds of limiting dilution cloning or its equivalent to meet the requirement of single cell origin. However, many legacy cell lines may not have been generated with process meeting this criteria potentially impeding the path to commercialization. A general monoclonality assessment strategy was developed based on using the site of plasmid integration for...
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  • Culturing Chinese hamster ovary cells on Cyclo Olefin Polymer triggers epithelial‐mesenchymal transition and spheroid formation, which increases the foreign gene expression driven by the MMLV‐LTR promoter

    7 days ago - By Wiley

    Abstract
    Chinese hamster ovary cells are frequently used for recombinant protein production as a host. While the RPP has been proven successful, there is still a compelling need for further improvement. Cyclo Olefin Polymer is a plastic material widely utilized due to its properties including its low protein absorption. We applied this as a raw material for RPP cell culture to see if the COP is suitable. A recombinant CHO cell line expressing the human erythropoietin gene under the control of the MMTV‐LTR was established. When the cells were cultured in a dish made from COP, the cells...
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  • Impact of iron raw materials and their impurities on CHO metabolism and recombinant protein product quality

    7 days ago - By Wiley

    Abstract
    Cell culture medium composition affects cell growth and critical quality attributes of monoclonal antibodies and recombinant proteins. One essential compound needed within the medium is iron because of its central role in many cellular processes. However, iron is also participating in Fenton chemistry leading to the formation of reactive oxygen species causing cellular damage. Therefore, this study sought to investigate the impact of iron in CCM on Chinese hamster ovary cell line performance, and CQAs of different recombinant proteins. Addition of either ferric ammonium citrate or...
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