• Protection of human induced pluripotent stem cells against shear stress in suspension culture by Bingham plastic fluid

    11 days ago - By Wiley

    Abstract
    Suspension culture is an important method used in the industrial preparation of pluripotent stem cells , for regenerative therapy and drug screening. Generally, a suspension culture requires agitation to keep PSC aggregates suspended and to promote mass transfer, but agitation also causes cell damage. In this study, we investigated the use of a Bingham plastic fluid, supplemented with a polysaccharide‐based polymer, to preserve PSCs from cell damage in suspension culture. Rheometric analysis showed that the culture medium gained yield stress and became a Bingham plastic fluid...
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  • Metabolic profiling of Chinese hamster ovary cell cultures at different working volumes and agitation speeds using spin tube reactors

    11 days ago - By Wiley

    Abstract
    Culture systems based on spin tube reactors have been consolidated in the development of manufacturing processes based on Chinese hamster ovary cells. Despite their widespread use, there is little information about the consequences of varying operational setting parameters on the culture performance of recombinant CHO cell lines. Here, we investigated the effect of varying working volumes and agitation speeds on cell growth, protein production, and cell metabolism of two clonally derived CHO cell lines (expressing an IgG1 and a “difficult‐to‐express” fusion protein)...
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