• Enhancing recombinant protein and viral vector production in mammalian cells by targeting the YTHDF readers of N6-methyladenosine in mRNA

    6 days ago - By Wiley

    Abstract
    N 6 -methyl adenosine is the most abundant internal modification on eukaryotic mRNA and has been implicated in a wide range of fundamental cellular processes. This modification is regulated and interpreted by a set of writer, eraser, and reader proteins. To date, there have been no reports on the potential of mRNA epigenetic regulators to influence recombinant protein expression in mammalian cells. In this study we evaluated the potential of manipulating the expression of the m6A YTH domain-containing readers, YTHDF1, 2, and 3 to improve recombinant protein yield based on their...
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