• Inhibition of E. coli host RNA Polymerase Allows Efficient Extracellular Recombinant Protein Production by Enhancing Outer Membrane Leakiness

    15 days ago - By Wiley

    Recombinant proteins in Escherichia coli are usually expressed inside the cell. With the growing interest in continuous cultivation, secretion of product to the medium is not only a benefit but a necessity in future bioprocessing. In this study, we show that induced decoupling of growth and heterologous gene expression in the E. coli X‐press strain, a strain derived from BL21, facilitates extracellular recombinant protein production. We investigated the effect of the process parameters temperature and specific glucose consumption rate on growth, productivity, lysis and leakiness, to find...
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