• Microscale Perfusion‐based Cultivation for Pichia pastoris Clone Screening Enables Accelerated and Optimized Recombinant Protein Production Processes

    9 days ago - By Wiley

    Pichia pastoris has emerged in the past years as a promising host for recombinant protein and biopharmaceutical production. In the establishment of high cell density fed‐batch biomanufacturing, screening phase and early bioprocess development still represent a bottleneck due to high‐cost and time‐consuming procedures as well as low experiment complexity. In the present work, we implement a screening protocol developed for P. pastoris clone selection in a multiplexed microfluidic device with 15 μL cultivation chambers able to operate in perfusion mode and monitor dissolved oxygen content in...
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