• Partial Purification and Characterization of Lipase from Locally Produced Edible Oil‐Seeds and its Relevance in Industries

    12 days ago - By Wiley

    Abstract
    Lipase was extracted from germinating seeds of Helianthus annus, Zea mays and Brassica compastris. The lipolytic activity was assessed using olive oil as substrate at different germination‐time and the maximum‐activity was obtained after 120 h. Partial‐purification was executed by precipitating the seed‐homogenate with varying concentration of ammonium‐sulfate solution. 80% ammonium sulfate solution showed maximum lipase activity of 5320IUmL −1 , 3500IUmL −1 , 3080IUmL −1 with 9.6, 6.9 and 4.8 fold purification and total‐protein‐content of 162 mg, 84 mg, and 60 mg for...
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