• Reduction of medium consumption in perfusion mammalian cell cultures using a perfusion rate equivalent concentrated nutrient feed

    10 days ago - By Wiley

    Abstract
    Media preparation for perfusion cell culture processes contributes significantly to operational costs and the footprint of continuous operations for therapeutic protein manufacturing. In this study, definitions are given for the use of a perfusion equivalent nutrient feed stream which, when used in combination with basal perfusion medium, supplements the culture with targeted compounds and increases the medium depth.
    Definitions to compare medium and feed depth are given in this paper. Using a concentrated nutrient feed, a 1.8‐fold medium consumption decrease and a 1.67‐fold...
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