• AFid: A tool for automated identification and exclusion of autofluorescent objects from microscopy images

    13 days ago - By Oxford Journals

    AbstractMotivationAutofluorescence is a long-standing problem that has hindered the analysis of images of tissues acquired by fluorescence microscopy. Current approaches to mitigate autofluorescence in tissue are lab-based and involve either chemical treatment of sections or specialized instrumentation and software to ‘unmix' autofluorescent signals. Importantly, these approaches are pre-emptive and there are currently no methods to deal with autofluorescence in acquired fluorescence microscopy images.ResultsTo address this, we developed Autofluorescence Identifier. AFid identifies...
    Read more ...