• CRISPR‐Cas9 Editing of CAFFEOYL SHIKIMATE ESTERASE 1 and 2 Shows Their Importance and Partial Redundancy in Lignification in Populus tremula x P. alba

    2 monthes ago - By Wiley

    SUMMARY
    Lignins are cell-wall-located aromatic polymers that provide strength and hydrophobicity to woody tissues. Lignin monomers are synthesized via the phenylpropanoid pathway, wherein CAFFEOYL SHIKIMATE ESTERASE converts caffeoyl shikimate into caffeic acid. Here, we explored the role of the two CSE homologs in poplar. Reporter lines showed that the expression conferred by both CSE1 and CSE2 promoters is similar. CRISPR-Cas9-generated cse1 and cse2 single mutants had a wild-type lignin level. Nevertheless, CSE1 and CSE2 are not completely redundant, as both single mutants accumulated...
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