• LINE‐1 vectors mediate recombinant antibody gene transfer by retrotransposition in Chinese hamster ovary cells

    5 days ago - By Wiley

    Abstract
    Background
    Retrotransposons, such as long interspersed element‐1 , can copy themselves to other genomic loci via a transposition event. Retrotransposons, therefore, have potential use as an efficient gene delivery tool to integrate multiple copies of a target gene into a host genome. Here, we developed a retrotransposon vector based on LINE‐1 that achieves target gene integration of multiple transgene copies.
    Methods
    The retrotransposon vector contains a neomycin resistance gene split by an intron as a marker gene, and a gene encoding an antibody single‐chain variable fragment...
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