• A single‐cell surgery microfluidic device for transplanting tumor cytoplasm into dendritic cells without nuclei mixing

    4 days ago - By Wiley

    Abstract
    This study aimed to demonstrate the feasibility of generating tumor cell vaccine models by single-cell surgery in a microfluidic device that integrates one-to-one electrofusion, shear flow reseparation, and on-device culture. The device was microfabricated from polydimethylsiloxane and consisted of microorifices for one-to-one fusion, and microcages for on-device culture. Using the device, we could achieve one-to-one electrofusion of leukemic plasmacytoid dendritic cells and Jurkat cells with a fusion efficiency of ∼80%. Fusion via the narrow microorifices allowed DC-like cells to...
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