• Field detection of multiple RNA viruses / viroids in apple using a CRISPR/Cas12a‐based visual assay

    20 days ago - By Wiley

    Summary
    Co‐infection of apple trees with several viruses/viroids is common and decreases fruit yield and quality. Accurate and rapid detection of these viral pathogens helps to reduce losses and prevent virus spread. Current molecular detection assays used for apple viruses require specialized and expensive equipment. Here, we optimized a Crispr/Cas12a‐based nucleic acid detection platform for the diagnosis of the most prevalent RNA viruses/viroid in apple, namely Apple necrotic mosaic virus , Apple stem pitting virus , Apple stem grooving virus , Apple chlorotic leaf spot virus and Apple...
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