• Exploring the Clinical Utility of Metagenomic Next-Generation Sequencing in the Diagnosis of Pulmonary Infection

    1 month ago - By Springer

    Abstract
    Introduction
    We aimed to explore the real-world clinical application value and challenges of metagenomic next-generation sequencing for pulmonary infection diagnosis.
    Methods
    We retrospectively reviewed the results of mNGS and conventional tests from 140 hospitalized patients with suspected pulmonary infections from January 2019 to December 2020. The sample types included bronchoalveolar lavage fluid, lung tissue by transbronchial lung biopsy, pleural effusion, blood, and bronchial sputum. Apart from the mNGS reports that our patients received, an extra comprehensive and thorough...
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  • Metagenomic Next-Generation Sequencing for the Diagnosis of Pneumocystis jirovecii Pneumonia in Non-HIV-Infected Patients: A Retrospective Study

    1 month ago - By Springer

    Abstract
    Introduction
    This study aimed to evaluate the utility of metagenomic next-generation sequencing for the diagnosis of Pneumocystis jirovecii pneumonia in non-human immunodeficiency virus-infected patients.
    Methods
    We conducted a retrospective study. A total of 60 non-human immunodeficiency virus-infected PJP patients and 134 patients diagnosed with non-PJP pneumonia were included. P. jirovecii and other co-pathogens identified by mNGS in bronchoalveolar lavage fluid and/or blood samples were analyzed. Using clinical composite diagnosis as the reference standard, we compared the...
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