• Modeling tangential flow filtration using reverse asymmetric membranes for bioreactor harvesting

    1 month ago - By Wiley

    Abstract
    Tangential flow filtration has many advantages for bioreactor harvesting as the permeate could be introduced directly to the subsequent capture step. However, membrane fouling has limited its widespread use. This is particularly problematic given the high cell densities encountered today. Here a reverse asymmetric membrane, where the more open surface faces the feed stream and the tighter barrier layer faces the permeate stream, has been investigated. The open surface contains pores up to 40 μm in diameter while the tighter barrier layer has an average pore size of 0.4 μm...
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  • Virus recovery by tangential flow filtration: A model to guide the design of a sample concentration process

    1 month ago - By Wiley

    Abstract
    A simple model is developed to describe the instantaneous and cumulative recovery of viruses from water during sample concentration by tangential flow filtration in the regime of constant water recovery, r
    . A figure of merit,
    M = r v
    r
    , is proposed as an aggregate performance metric that captures both the efficiency of virus recovery and the speed of sample concentration. We derive an expression for virus concentration in the sample as a function of filtration time with the rate‐normalized virus loss, η=1−rvr, as a parameter. A practically relevant case is considered when the...
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