• Sensitive and specific detection of Xanthomonas campestris pv. zinniae by PCR using pathovar-specific primers

    24 days ago - By Springer

    Abstract
    A sensitive and specific assay was developed to detect bacterial leaf and flower spot disease on zinnia caused by Xanthomonas campestris pv. zinniae. A draft genome sequence was determined for Xcz strain E4, isolated from zinnia in Jilin province, China. Comparative genomics analyses were utilized to identify a sequence specific to Xcz in the unique gene 0834. This sequence region was amplified as 1044 bp DNA fragment using designed primers 0834F and 0834R. Using this primer set, specific PCR products were only amplified from DNA from Xcz strains and not from other pathovars of X...
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