• Base editing with high efficiency in allotetraploid oilseed rape by A3A‐PBE base editing system

    25 days ago - By Wiley

    Summary
    CRISPR/Cas‐ base editing is an emerging technology that could convert a nucleotide to another type at the target site. In this study, A3A‐PBE system consisting of human A3A cytidine deaminase fused with a Cas9 nickase and uracil glycosylase inhibitor, was established and developed in allotetraploid Brassica napus. We designed three sgRNAs to target ALS , RGA and IAA7 genes, respectively. Base editing efficiency was demonstrated to be more than 20% for all of the three target genes. Target sequencing results revealed that the editing window ranged from C1 to C10 of the PAM sequence...
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