• Engineering herbicide resistance via prime editing in rice

    2 monthes ago - By Wiley

    Abstract
    Although CRISPR‐Cas9 has revolutionized our ability to generate site‐specific double‐strand breaks, precise editing of the genome remains challenging in most eukaryotes, including plants. In plants homology‐directed repair is inefficient, limiting our ability to make precise edits of the DNA sequence. Moreover, cytosine and adenine base editors have serious drawbacks including lower efficiency, unclean edited sequence, and the possibility of off‐target mutations at other loci. Chimeric single guide RNAs can provide editing information, in RNA form, but this modality suffers from...
    Read more ...