• Culturing Chinese hamster ovary cells on Cyclo Olefin Polymer triggers epithelial‐mesenchymal transition and spheroid formation, which increases the foreign gene expression driven by the MMLV‐LTR promoter

    1 month ago - By Wiley

    Abstract
    Chinese hamster ovary cells are frequently used for recombinant protein production as a host. While the RPP has been proven successful, there is still a compelling need for further improvement. Cyclo Olefin Polymer is a plastic material widely utilized due to its properties including its low protein absorption. We applied this as a raw material for RPP cell culture to see if the COP is suitable. A recombinant CHO cell line expressing the human erythropoietin gene under the control of the MMTV‐LTR was established. When the cells were cultured in a dish made from COP, the cells...
    Read more ...