• Micropipette Aspiration‐Based Assessment of Single Channel Water Permeability

    1 month ago - By Wiley

    The method works at low reconstitution densities of water conducting channels and is not limited by unstirred layers. It allows for simultaneous measurements of channel abundance by fluorescence correlation spectroscopy and visualization of inhibitor effects without changing the sample.
    Abstract
    Measurements of the unitary hydraulic conductivity of membrane channels, p f , may be hampered by difficulties in producing sufficient quantities of purified and reconstituted proteins. Low yield expression, the purely empiric choice of detergents, as well as protein aggregation and misfolding...
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