• Novel Promoters Derived from Chinese Hamster Ovary Cells via In Silico and In Vitro Analysis

    28 days ago - By Wiley

    CHO endogenous promoters and enhancers are identified using histone marks and chromatin states, ranked based on expression level and tested for normalized promoter strength. In an initial screen within stable cell lines, the strongest CHO promoter appears to be more stable than the human cytomegalovirus promoter with enhancer, making it a promising candidate for recombinant protein production and engineering applications.
    For the industrial production of recombinant proteins in mammalian cell lines, a high rate of gene expression is desired. Therefore, strong viral promoters are commonly...
    Read more ...