• Mammalian cell cultivation using nutrients extracted from microalgae

    1 day ago - By Wiley

    Abstract
    Mammalian cells have been used in various research fields. More recently, cultured cells have been used as the cell source of “cultured meat.” Cell cultivation requires media containing nutrients, of which glucose and amino acids are the essential ones. These nutrients are generally derived from grains or heterotrophic microorganisms, which also require various nutrients derived from grains. Grain culture, in turn, requires many chemical fertilizers and agrochemicals, which can cause greenhouse gas emission and environmental contamination. Furthermore, grain production is greatly...
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  • SH‐SY5Y and LUHMES cells display differential sensitivity to MPP+, tunicamycin and epoxomicin in 2D and 3D cell culture

    1 day ago - By Wiley

    Abstract
    SH‐SY5Y and LUHMES cell lines are widely used as model systems for studying neurotoxicity. Most of the existing data regarding the sensitivity of these cell lines to neurotoxicants have been recorded from cells growing as two‐dimensional cultures on the surface of glass or plastic. With the emergence of three‐dimensional culture platforms designed to better represent native tissue, there is a growing need to compare the toxicology of neurons grown in 3D environments to those grown in 2D to better understand the impact that culture environment has on toxicant sensitivity. Here, a...
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