• SMRT Sequencing Helps Crack the Code of Elusive Centromeres

    2 monthes ago - By Pacific Biosciences

    Crucial assembly sites and mitosis mediators, centromeres are central to every cell, but missing from even the most complete genome assemblies.
    Until now.
    In a PLOS Biology paper , Amanda Larracuente and colleagues at the University of Rochester and Barbara G. Mellone of the University of Connecticut, described how they sequenced the repetitive regions of the fruit fly genome, including its centromeres, using SMRT Sequencing.
    Embedded in blocks of highly repetitive satellite DNA, centromeres have eluded efforts at assembly.
    Only recently, long-read single molecule sequencing technologies...
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