• Episo: quantitative estimation of RNA 5-methylcytosine at isoform level by high-throughput sequencing of RNA treated with bisulfite

    8 days ago - By Oxford Journals

    AbstractMotivationRNA 5-methylcytosine is a type of post-transcriptional modification that may be involved in numerous biological processes and tumorigenesis. RNA m 5 C can be profiled at single-nucleotide resolution by high-throughput sequencing of RNA treated with bisulfite. However, the exploration of transcriptome-wide profile and potential function of m 5 C in splicing remains to be elucidated due to lack of isoform level m 5 C quantification tool.ResultsWe developed a computational package to quantify Epitranscriptomal RNA m 5 C at the transcript isoform level. Episo consists of...
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  • Schex avoids overplotting for large single cell RNA-sequencing datasets

    8 days ago - By Oxford Journals

    AbstractSummaryDue to the scale and sparsity of single cell RNA-sequencing data, traditional plots can obscure vital information. Our R package schex overcomes this by implementing hexagonal binning, which has the additional advantages of improving speed and reducing storage for resulting plots.Availabilityschex is freely available from Bioconductor via and its development version can be accessed on GitHub via .
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