• Overexpression of a developing xylem cDNA library in transgenic poplar generates high mutation rate specific to wood formation

    8 days ago - By Wiley

    Abstract
    We investigated feasibility of the FOX (Full‐length complementary DNA OvereXpression) system as a mutagenesis approach in poplar, using developing xylem tissue. The main goal was to assess the overall mutation rate and if the system will increase instances of mutants affected in traits linked to the xylem tissue. Indeed, we found a high mutation rate of 17.7%, whereas 80% of all mutants were significantly affected in cellulose, lignin, and/or hemicellulose. Cell wall biosynthesis is a major process occurring during xylem development. Enrichment of mutants affected in cell wall...
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